grna library Search Results


mouse lentiviral crispr guide rna library v2  (Addgene inc)
93
Addgene inc mouse lentiviral crispr guide rna library v2
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Mouse Lentiviral Crispr Guide Rna Library V2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse lentiviral crispr guide rna library v2/product/Addgene inc
Average 93 stars, based on 1 article reviews
mouse lentiviral crispr guide rna library v2 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
human genome wide library  (Addgene inc)
94
Addgene inc human genome wide library
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Human Genome Wide Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human genome wide library/product/Addgene inc
Average 94 stars, based on 1 article reviews
human genome wide library - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
alt rtm crispr system  (Danaher Inc)
91
Danaher Inc alt rtm crispr system
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Alt Rtm Crispr System, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alt rtm crispr system/product/Danaher Inc
Average 91 stars, based on 1 article reviews
alt rtm crispr system - by Bioz Stars, 2026-04
91/100 stars
  Buy from Supplier
pre amplified  (Addgene inc)
92
Addgene inc pre amplified
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Pre Amplified, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pre amplified/product/Addgene inc
Average 92 stars, based on 1 article reviews
pre amplified - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
library backbone plasmid  (Addgene inc)
86
Addgene inc library backbone plasmid
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Library Backbone Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/library backbone plasmid/product/Addgene inc
Average 86 stars, based on 1 article reviews
library backbone plasmid - by Bioz Stars, 2026-04
86/100 stars
  Buy from Supplier
custom grna library  (GenScript corporation)
90
GenScript corporation custom grna library
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Custom Grna Library, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom grna library/product/GenScript corporation
Average 90 stars, based on 1 article reviews
custom grna library - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
grna library  (Twist Bioscience)
90
Twist Bioscience grna library
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Grna Library, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/grna library/product/Twist Bioscience
Average 90 stars, based on 1 article reviews
grna library - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
human kinome grna library lentivirus  (Vigene Biosciences)
90
Vigene Biosciences human kinome grna library lentivirus
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Human Kinome Grna Library Lentivirus, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human kinome grna library lentivirus/product/Vigene Biosciences
Average 90 stars, based on 1 article reviews
human kinome grna library lentivirus - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
second-generation “geko” grna library  (Broad Institute Inc)
90
Broad Institute Inc second-generation “geko” grna library
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Second Generation “Geko” Grna Library, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/second-generation “geko” grna library/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
second-generation “geko” grna library - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
pooled grna libraries were synthesized as single-stranded oligonucleotides  (Twist Bioscience)
90
Twist Bioscience pooled grna libraries were synthesized as single-stranded oligonucleotides
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Pooled Grna Libraries Were Synthesized As Single Stranded Oligonucleotides, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pooled grna libraries were synthesized as single-stranded oligonucleotides/product/Twist Bioscience
Average 90 stars, based on 1 article reviews
pooled grna libraries were synthesized as single-stranded oligonucleotides - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
the crispr grna screening library  (Illumina Inc)
90
Illumina Inc the crispr grna screening library
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
The Crispr Grna Screening Library, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/the crispr grna screening library/product/Illumina Inc
Average 90 stars, based on 1 article reviews
the crispr grna screening library - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
grna library synthesis oligonucleotide pools  (CustomArray Inc)
90
CustomArray Inc grna library synthesis oligonucleotide pools
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Grna Library Synthesis Oligonucleotide Pools, supplied by CustomArray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/grna library synthesis oligonucleotide pools/product/CustomArray Inc
Average 90 stars, based on 1 article reviews
grna library synthesis oligonucleotide pools - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a lentiviral CRISPR-library. Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.

Journal: bioRxiv

Article Title: Genome wide CRISPR screen for Pasteurella multocida toxin (PMT) binding proteins reveals LDL Receptor Related Protein1 (LRP1) as crucial cellular receptor

doi: 10.1101/2022.08.04.502755

Figure Lengend Snippet: Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a lentiviral CRISPR-library. Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.

Article Snippet: We utilized a well-functioning and validate genome-wide mouse lentiviral CRISPR guide RNA library v2 (Addgene).

Techniques: Stable Transfection, Expressing, Transduction, CRISPR, Amplification

The volcano plot shows gene expression changes between cells treated with lethal PMT-DTa chimera and those only transduced with the CRISPR library. All reads were mapped locally using BWA-MEM [ ,6], then quantified with featureCounts [4], and finally fold changes between the condition were calculated by DESeq2 [7] (see galaxy history). The volcano plot was drawn with the bioinfokit toolkit [8]. Significantly enriched genes, having a positive fold change above 0.584 and a p-value lower or equal 5%, are shown in blue. The significance thresholds are marked by gray-dotted lines. The ten most significant genes are highlighted with their name. Non-significant genes are colored in gray.

Journal: bioRxiv

Article Title: Genome wide CRISPR screen for Pasteurella multocida toxin (PMT) binding proteins reveals LDL Receptor Related Protein1 (LRP1) as crucial cellular receptor

doi: 10.1101/2022.08.04.502755

Figure Lengend Snippet: The volcano plot shows gene expression changes between cells treated with lethal PMT-DTa chimera and those only transduced with the CRISPR library. All reads were mapped locally using BWA-MEM [ ,6], then quantified with featureCounts [4], and finally fold changes between the condition were calculated by DESeq2 [7] (see galaxy history). The volcano plot was drawn with the bioinfokit toolkit [8]. Significantly enriched genes, having a positive fold change above 0.584 and a p-value lower or equal 5%, are shown in blue. The significance thresholds are marked by gray-dotted lines. The ten most significant genes are highlighted with their name. Non-significant genes are colored in gray.

Article Snippet: We utilized a well-functioning and validate genome-wide mouse lentiviral CRISPR guide RNA library v2 (Addgene).

Techniques: Gene Expression, Transduction, CRISPR